Entries by Katy

An excitation-reception collinear probe for ultrasonic, photoacoustic, and thermoacoustic tri-modal volumetric imaging

Imaging systems that integrate multiple modalities can reveal complementary anatomic and functional information as they exploit different contrast mechanisms, which have shown great application potential and advantages in preclinical studies. A portable and easy-to-use imaging probe will be more conducive to transfer to clinical practice. Here, we present a tri-modal ultrasonic (US), photoacoustic (PA), and […]

Ultraselective Carbon Nanotubes for Photoacoustic Imaging of Inflamed Atherosclerotic Plaques

Disruption of vulnerable atherosclerotic plaques often leads to myocardial infarction and stroke, the leading causes of morbidity and mortality in the United States. A diagnostic method that detects early-stage high-risk atherosclerotic plaques could prevent these sequelae. The abundant immune cells in the arterial wall, especially inflammatory Ly-6Chi monocytes and foamy macrophages, are indicative of plaque inflammation, […]

Sarcoma Molecular Pathological Diagnosis of FFPE Tissues without Dewaxing based on Lipidomic Proles using SpiderMass Technology

Background :Formalin-xation and paran-embedding (FFPE) is the worldwide gold standard for tissue preservation and routine pathology for general and cancer diagnostics. Despite robust histopathology methods, accurate diagnostic remains dicult for certain cases. Overall, the entire process is timeconsuming, labor-intensive and doesn’t reach the right sensitivity and specicity. Lipids are central players in cancer, but they […]

Optical Microscopy-Guided Laser Ablation Electrospray Ionization Ion Mobility Mass Spectrometry: Ambient Single Cell Metabolomics with Increased Confidence in Molecular Identification

Single cell analysis is a field of increasing interest as new tools are continually being developed to understand intercellular differences within large cell populations. Laser-ablation electrospray ionization mass spectrometry (LAESI-MS) is an emerging technique for single cell metabolomics. Over the years, it has been validated that this ionization technique is advantageous for probing the molecular […]

Quantitative Imaging Analysis of the Spatial Relationship between Antiretrovirals, Reverse Transcriptase Simian-Human Immunodeficiency Virus RNA, and Collagen in the Mesenteric Lymph Nodes of Nonhuman Primates

Human immunodeficiency virus (HIV) persistence in tissue reservoirs is a major barrier to HIV cure. While antiretrovirals (ARVs) suppress viral replication, antiretroviral therapy (ART) interruption results in rapid rebound viremia that may originate from lymphoid tissues. To understand the relationship between anatomic distribution of ARV exposure and viral expression in lymph nodes, we performed mass […]

Investigations of β-carotene radical cation formation in infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

Radical cationization of endogenous hydrocarbons in cherry tomatoes was previously reported using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI), a mass spectrometry imaging technique that operates at ambient conditions and requires no sample derivatization. Due to the surprising nature of this odd-electron ionization, subsequent experiments were performed on β-carotene to determine the amount of radical […]

Three-dimensional (3D) imaging of lipids in skin tissues with infrared matrix-assisted laser desorption electrospray ionization (MALDESI) mass spectrometry

Three-dimensional (3D) mass spectrometry imaging (MSI) has become a growing frontier as it has the potential to provide a 3D representation of analytes in a label-free, untargeted, and chemically specific manner. The most common 3D MSI is accomplished by the reconstruction of 2D MSI from serial cryosections; however, this presents significant challenges in image alignment […]

Rapid LA-REIMS and comprehensive UHPLC-HRMS for metabolic phenotyping of feces

Ambient ionization-based techniques hold great potential for rapid point-of-care applicable metabolic fingerprinting of tissue and fluids. Hereby, feces represents a unique biospecimen as it integrates the complex interactions between the diet, gut microbiome and host, and is therefore ideally suited to study the involvement of the diet-gut microbiome axis in metabolic diseases and their treatments […]

Dating a Martian meteorite with 20 Myr precision using a prototype in-situ dating instrument

We are continuing the development of a compact resonance ionization mass spectrometer for geochronology of planetary specimens, ultimately for in-situ use on the Moon or Mars. We previously demonstrated 87Rb–87Sr isochrons for the Martian meteorite Zagami and the Duluth Gabbro with a best precision for Zagami of 100 Myr, using 10-ns, 250-μJ laser ablation pulses […]